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Be careful using the sharp knife. An adult may need to help with this. Blend on high speed, pulsing when necessary, until the liver is smooth and no chunks are present. Be careful of the sharp blades in the blender. To the blended liver drop, add one drop of hydrogen peroxide.
You should see a lot of bubbles! What do you think the bubbles are made of? This shows that the liver enzyme catalase is working to start the chemical reaction that breaks down the hydrogen peroxide that would be harmful to the body into less dangerous compounds.
What is the color and consistency of this mixture? Put one drop of the mixture on a clean part of the large plate and add one drop of hydrogen peroxide to it. Compared with the untreated blended liver, did more, less or about the same amount of bubbles form? Did they form more slowly, more quickly or at about the same rate?
Did more, less or about the same amount of bubbles form? Cover the bowl and microwave it on high for 20 seconds. How does the blended liver look after heating?
Remove a drop-size amount of the heated liver and put it on a clean part of the large plate. Add one drop of hydrogen peroxide to it. Which condition s makes it work the worst? Why do you think this is so?
Try experimenting with other conditions. Or you could try adding more than one teaspoon of vinegar or baking soda and then test the enzyme.
Under which conditions does the enzyme work well, and under which ones does it work poorly? You could try this activity again using another enzyme, called bromelain, which digests proteins and can be extracted from pineapples. One protein that is fun to digest using bromelain is gelatin, which is found in many puddings and gelatinous desserts.
How do different conditions affect the ability of bromelain to digest proteins? Observations and results When exposed to hydrogen peroxide, did the blended liver bubble less when mixed with either the vinegar or baking soda compared with when it was untreated?
Did it bubble even less after it was microwaved? An enzyme needs certain conditions to work, and the ideal environment can be a hint as to where the enzyme normally works in the body.
And because different body tissues have distinct environments—acidic or warm—each enzyme is tuned to work best under specific conditions.
Different tissues in the body have different pHs pH is a measure of how basic or acidic a solution is. The liver maintains a neutral pH about pH 7which is easiest for its enzymes, such as catalase, to work in.
Consequently, when exposed to hydrogen peroxide the liver should have produced more bubbles oxygen gasand at a faster rate, when it was untreated than when exposed to vinegar or baking soda.
It may have bubbled more when treated with baking soda, compared with vinegar, because it might have been better able to return the pH to around 7.
Similarly, enzymes in the liver are also used to functioning at body temperature 37 degrees Celsiusso microwaving the blended liver to a temperature hotter than that should have damaged the catalase enzyme and clearly decreased the amount of bubbles when it was exposed to hydrogen peroxide.
Cleanup Safely dispose of any raw liver meat used in this activity by putting it in the trash when you are done. Completely disinfect any surfaces that the raw liver meat touched during this activity, and be sure to thoroughly wash your hands with soap and warm water.Manifest Cliches and expressions give us many wonderful figures of speech and words in an analysis of cultural identity in genre films the English the reaction of catalase in varioius substance language.
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